Season 2 of Odd Squad started airing in 2016 with two new main characters, Agent Olympia and Agent Otis, replacing Olive and Otto in Ms. O's Squad, as well as new scientist Agent Oona replacing Agent Oscar six episodes in. It began airing on June 20, 2016, with a special entitled "Odd Squad: Agents of Change" which featured "O is Not For Over" and "First Day" back to back.
The website was also updated for the second season with new games, wall of agents, and character videos.
In the second season, Olive and Otto leave to run their own squad, and Ms. O hires two new agents in their place, an academy grad Olympia, and mysterious agent Otis. After Odd Squad defeats Odd Todd in "O is not for Over", headquarters gets a new look featuring an expanded lab, new desks, and the all-new creature room, run by Agent Ocean. Other new rooms include the Task Force room, Coach O's Office, and Ogg's room (Introduced in "Olympia's Day"). Todd becomes a gardener. Oscar hires a new lab assistant, Oona who, after Oscar becomes president of the academy (after the current president tried to take over the world in "Oscar Strikes Back"), takes over as lab director. Oscar's voice is also deeper from the first episode of season 2 until the final episode that he's in.
Oona also takes over Oscar's role in the Training Videos and Other Interstitials.
In "Odd Squad: The Movie", Oscar, Olive and Otto return as guests. While not being officially confirmed, it is likely that Octavia, Oren and Olaf have also left the series.
- First Day (Aired: June 20, 2016)
- Back to the Past/Odd Squad Needs You (Aired: June 21, 2016)
- Mid-Day in the Garden of Good and Odd/Failure to Lunch (Aired: June 23, 2016)
- The O Team/Show Me the Money (Aired: June 27, 2016)
- Flawed Squad/The Creature Whisperer (Aired: June 28, 2016) (Production Code: 108)
- Oscar Strikes Back (Aired: July 5, 2016) (Production Code: 105)
- Olympia's Day/Otis's Day(Aired: July 6, 2016) (Production Code: 106)
- And Then They Were Puppies/A Case of the Sillies (Aired: July 7, 2016) (Production Code: 107)
- Happy Halfiversary/Good Egg Bad Egg (Aired: July 8, 2016)
- Night Shift/Put Me in Coach (Aired: July 11, 2016)
- Extreme Cakeover/A Job Well Undone (Aired: November 7, 2016)
- Three's Company/Behind Enemy Mimes (Aired: November 8, 2016)
- Drop Gadget Repeat/20 Questions (Aired: November 9, 2016)
- High Maintenance/Not OK Computer (Aired: November 10, 2016)
- O is For Opposite/Agent Oksana's Kitchen Nightmares (Aired: March 20th, 2017)
- Rookie Night/Who Let the Doug Out? (Aired: March 21st, 2017)
- The Cherry On Top inator/Sir (Aired: March 22nd, 2017)
- Orchid's Almost Half Hour Talent Show/The Perfect Score (Aired July 17, 2017)
- The Voice/Partner Problems (Aired July 18, 2017)
- Oona and the Oonabots/The Ninja Situation (Aired July 19, 2017)
- The Breakfast Club/Dr. O: Party Time, Excellent (Aired July 20, 2017)
- Haunt Squad/Safe House in the Woods (Airing: October 16, 2017)
- Xs and Os/Dr. O No (Airing: October 17, 2017)
- Negative Town/License to Science (Airing: October 18, 2017)
- Two Agents and a Baby/Ocean and the Fly (Airing: October 19, 2017)